GV344

Vector information:

General:12375 bp

HIV-1_5_LTR,truncHIV-1_3_LTR:835-1015

hU6Promoter:2602-2869

Ubiquitin Promoter:2957-4173

firefly_Luciferase:4216-5868

IRES:5878-6462

Puromycin:6469-7068

HIV-1_5_LTR,truncHIV-1_3_LTR:8180-8360

Poly A:8392-8616

pBR322_origin:11227-10608

Ampicillin:12242-11382

 

Primer locations and sequences:

H1-F(2479-2502):GGAAAGAATAGTAGACATAATAGC

UBI-R-2(3150-3132):AGTCCCGTCCTAAAATGTC

 

说明:由于载体图谱空间有限,部分元件未在图谱中体现