Vector information:
General:4731bp
PCMV IE:1- 589
EGFP:613- 1329
MCS:1330- 1417
SV40 poly A:1550- 1600
f1 ori:1647- 2102
Bacterial promoter for expression of Kan gene:2164- 2192
SV40 Enhancer:2276- 2419
SV40 early promoter:2276- 2544
SV40 origin:2443- 2578
Kanamycin/neomycin:2627- 3421
HSV TK poly A:3657- 3675
pUC ori:4006- 4649
Primer locations and sequences:
pEGFP-C-5(1266- 1287):CATGGTCCTGCTGGAGTTCGTG
SV40pA-R(1575- 1556):GAAATTTGTGATGCTATTGC
说明:由于载体图谱空间有限,部分元件未在图谱中体现
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