GV144

Vector information:

General:4731bp

PCMV IE:1- 589

EGFP:613- 1329

MCS:1330- 1417

SV40 poly A:1550- 1600

f1 ori:1647- 2102

Bacterial promoter for expression of Kan gene:2164- 2192

SV40 Enhancer:2276- 2419

SV40 early promoter:2276- 2544

SV40 origin:2443- 2578

Kanamycin/neomycin:2627- 3421

HSV TK poly A:3657- 3675

pUC ori:4006- 4649

 

Primer locations and sequences:

pEGFP-C-5(1266- 1287):CATGGTCCTGCTGGAGTTCGTG

SV40pA-R(1575- 1556):GAAATTTGTGATGCTATTGC

 

说明:由于载体图谱空间有限,部分元件未在图谱中体现